Table 1.
Cryo-EM data collection, refinement, and validation statistics
| Data collection and processing | |
|---|---|
| Magnification | 36,764 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 24 |
| Defocus range (μm) | −1.4 to −3.2 |
| Pixel size (Å) | 0.68 (Super-resolution mode) |
| Symmetry imposed | C2 |
| Initial particle images (no.) | 1,729,419 |
| Final particle images (no.) | 184,561 |
| Map resolution (Å) | 3.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3–5 |
| Refinement | |
| Model resolution cutoff (Å) | 3.9 |
| FSC threshold | 0.143 |
| Map sharpening B-factor (Å2) | −240 |
| Model composition | |
| Non-hydrogen atoms | 7344 |
| Protein residues | 948 (474 per protomer) |
| Ligands | 0 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.288 |
| Validation | |
| MolProbity score | 2.11 |
| Clashscore | 9.23 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 86.99 |
| Allowed (%) | 13.01 |
| Disallowed (%) | 0 |