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. 2018 Mar 2;9(3):357. doi: 10.1038/s41419-018-0392-5

Fig. 5. The identification of Rab27a KO mouse and the ability of exosomes secretion.

Fig. 5

Construction of Rab27a KO mice model by TALEN genome-editing technologies; the coat color phenotype of wild type (WT) and Rab27a KO mice (a), and the diagram of TALEN target sites (b). c The level of Rab27a protein in cardiac tissues of WT and KO mice were analyzed by western blot; GAPDH was employed as a loading control. Quantitative data of Rab27a band densities were shown (d). e, f Quantitative data of exosomes released from cardiomyocytes and cardiac tissues. g TEM micrograph of exosomes in cardiac tissues, the arrows indicated the typical exosomes. Scale bar, 100 nm. *p < 0.05 versus Rab27a+/+. #p < 0.05 versus WT. All values expressed as mean ± SD, n = 4 for each group