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. 2018 Mar 2;8:3947. doi: 10.1038/s41598-018-22289-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN contributes to the G2/M checkpoint. (A) DU145 cells were transfected with either scrambled (CTR) or PTEN (siPTEN) siRNA. After 48 h, cells were UV-irradiated (50 J/m²) and the CHK1 auto-phosphorylation site (S296) was immunoblotted. Both ß-actin and CHK1 were used as loading controls. (B) Percent of DU145 cells in G2-phase was monitored using propidium iodide (PI) staining by flow cytometry after treatment with scRNA, siPTEN or the CHK1 inhibitor UCN01 (50 nM). Asterisk (*) represents significant difference (P < 0.05). (C) After the indicated treatments, DU145 cells were irradiated with 10 Gy, treated with colcemid to accumulate mitotic cells and then co-stained with phosphohistone H3 (S10) antibody and PI to detect mitotic cells. Shown are the means ± SEM from three experiments. (D) Immunofluorescence microscopy of CHK1 2 h after 2 Gy in siRNA-treated DU145 cells. (E) Quantitation of subcellular localization of CHK1 presented in D. Shown are the means ± SEM from at least three different experiments. ns: not statistically significant. (F) Western blot showing pCHK1-S280 and CHK1 in DU145 transfected with siCTR or siPTEN. Band intensities (measured by UNSCAN-IT gel V6.1) in percentage are shown.