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. 2018 Mar 2;8:3907. doi: 10.1038/s41598-018-22326-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stain index of enriched surface proteins in MSC-PLT. (a) Representative contour plot of paired MSC samples with MSC-PLT displaying smaller size and lower complexity than MSC-FCS. MSC-FCS showed significantly higher background fluorescence, herein defines as median fluorescence intensity (MFI), in the APC channel, than MSC-PLT. (b) Stain index of 13 surface proteins enriched on MSC-PLT, in comparison with MSC-FCS (n = 3); error bars represent mean ± SEM and *p-value < 0.05. (c) Fold increase in stain index shown as MSC-PLT relative to MSC-FCS). Red line represents a fold increase of 1.5.