Fig. 4. C10 blocks autophagic flux in MCF-7 cells.
a Representative western blot of LC3B and p62/SQSTM1 protein level in MCF-7 cells cultured in full or serum depleted medium or medium containing IC50 dose of C10 or 10 µM chloroquine for 24 h (1 day) followed by n days of culture in drug free full medium (1 + n). b Representative confocal images showing colocalization of mCherry and GFP signals in cells transiently transfected with plasmid coding for mCherry-GFP-LC3B and treated as described above (one of 4 independent experiments). Scale bars: 10 µm. CQ chloroquine, Starv starvation