Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 2;8:3912. doi: 10.1038/s41598-018-22208-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SESN2 is negatively regulated by AA in the process of AA-mediated regulation of cell proliferation and casein synthesis in CMEC. (A) Cell proliferation of CMEC treated with EAA or AA was analyzed by MTT assay, the data of “AA− group” was defined as “1”; (B) Cell cycle of CMEC treated with EAA or AA was analyzed by FC; (C) Expression of casein in CMEC treated with EAA or AA was analyzed by WB, the ratio value of “AA− group” was defined as “1”; (D) Expression of proteins related mTORC1 pathway, SESN2 and GCN2, and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with EAA or AA was analyzed by WB, the ratio value of “AA− group” was defined as “1”; (E) Subcellular localization of mTOR in CMEC treated with EAA or AA for 6 h was analyzed by IF. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant difference (p < 0.05). Uncropped images for all Western blots can be found in Supplementary Fig. 4.