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. 2018 Mar 2;8:3912. doi: 10.1038/s41598-018-22208-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SESN2 negatively regulates AA-mediated mTORC1 activation in CMEC. (A) Expression of proteins related mTORC1 pathway and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with si-SESN2 was analyzed by WB, the ratio value of “B group” was defined as “1”; (B) Expression of proteins related mTORC1 pathway and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with SESN2 GO was analyzed by WB, the ratio value of “B group” was defined as “1”; (C) Expression of proteins related mTORC1 pathway and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with AA−, AA−/si-SESN2, EAA+, and AA+ was analyzed by WB, the ratio value of “AA− group” was defined as “1”; (D) Expression of proteins related mTORC1 pathway and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with AA−, EAA+, AA+, EAA+/SESN2 GO, and AA+/SESN2 GO was analyzed by WB, the ratio value of “AA− group” was defined as “1”. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant differences (p < 0.05). B: Cells were no transfected; NC: Cells were transfected with negative control siRNA; EV: Cells were transfected with empty vector. Uncropped images for all Western blots can be found in Supplementary Fig. 4.