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. 2018 Mar 2;8:3936. doi: 10.1038/s41598-018-22012-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth kinetics of the Rv3484 deletion mutant strain in 7H9 medium and acid fast properties. (a) Bacteria were grown in fully supplemented 7H9 medium and OD₆₀₀ measured at indicated time points. Means ± SD of biological triplicates performed in technical duplicates are shown. (b) Acid fast Ziehl-Neelsen staining of Mtb H37Rv, Mtb H37RvΔRv3484, Mtb H37RvΔRv3484::Rv3484 as well as H37RvΔRv3484::pMV306 was performed according to standard procedures (1000× magnification, scale ≙ 10 µm).