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. 2018 Mar 2;8:3936. doi: 10.1038/s41598-018-22012-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histopathology of lung sections of mice infected with the Mtb H37Rv Rv3484 deletion mutant. HE stained sections of lungs prepared at day 21 (a), 42 (b), 90 (c) and 180 (d) post-infection scanned with 1× (original scan area 1 cm × 1 cm) using a slide scanner and 100 × (scale ≙ 100 µm) magnification of wild type (H37Rv; confirmed infection dose 88 CFU/lung), the Rv3484 deletion mutant (H37Rv∆Rv3484; confirmed infection dose 210 CFU/lung) and the complemented mutant strain (H37Rv∆Rv3484::Rv3484; confirmed infection dose 171 CFU/lung).