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. 2018 Feb 9;115(9):2090–2095. doi: 10.1073/pnas.1716161115

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Fig. 3. — CTFR-bESCs show molecular signatures characteristic of primed pluripotency. (A) Transcriptome analysis of selected naive and primed pluripotency markers in CTFR-bESCs. RNA-seq was performed, and RPKM values were used to define expressed (RPKM ≥0.4; red) and nonexpressed (RPKM <0.4; green) genes. The means of two biological replicates are shown. (B) Genome browser snapshots of histone methylation profiles of primed and naive pluripotency markers in CTFR-bESCs. (C) Genome browser snapshots of H3K4me3 and H3K27me3 marks in core pluripotency genes (POU5F1, SOX2, NANOG, SALL4) in CTFR-bESCs.