Fig. 3.

STING decreases infection probability. (A) The MEFs indicated were infected with VSV-LUC at MOI 1.0. Infection was monitored by luciferase assay at the indicated time points. (B) Virus growth rates [ln((Luciferase units)/hour] were determined from the exponential phase of the data in A by linear regression and are shown as the best fit values with 95% confidence intervals. Growth rates were compared using the Mann–Whitney U test. (C) Plaque forming assays were performed on the MEFs indicated. Plaques were counted by fluorescence microscopy, and a viral titer (pfu/mL) was calculated. Relative plaque efficiency was calculated from the fold increase in titer calculated on shCTRL and shSTING cells. Data are represented as mean ± SEM. (D) The MEFs indicated were transfected in the presence of lipofectamine 2000 or mock-transfected with purified VSV-LUC cores. Luciferase was quantified 5 h after transfection by luciferase assay. Data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.0001 (Student’s t test).