Fig. 1.
Identifying exosomal microRNAs markedly secreted by osteoblastic phenotype-inducing cancer cell lines. (A) Representative X-ray images and H&E stains of tibiae at 2 wk after the intratibial injection of 1 × 106 tumor cells into NOD/SCID mice. Several osteoclasts (arrowheads) were observed in the osteolytic lesions. (Scale bars, 20 μm.) (B) The expression profile of miRNAs in exosomes secreted by cancer cell lines. Exosomes from noncancerous cells (HEK293) were used as a normalization control. Red color denotes higher expression, and blue color denotes lower expression relative to the control.