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. 2018 Feb 12;115(9):2204–2209. doi: 10.1073/pnas.1717363115

Fig. 2.

Fig. 2.

hsa-miR-940 promotes the osteogenic differentiation of human mesenchymal stem cells. (A) Confocal microscopy images showing that Venus-labeled C4-2B exosomes were incorporated into hMSCs (Center). qPCR analysis showing the increased expression of ALPL in hMSCs cultured with C4-2B exosomes (Right). (Scale bars, 10 μm.) (B and C) The effects of transient overexpression of cancer-secreted miRNAs on the osteogenic differentiation of hMSCs. ALP activity assay (B) and von Kossa staining (C) showed that miR-940 overexpression induced the osteogenic differentiation. n.d., not detected. (Scale bars, 4 mm.) (D) qPCR analysis showing higher expression of ALPL in miR-940–overexpressing hMSCs after 6-d osteogenic induction. All of the data are the means ± SEMs (n = 3). n.s., not significant, *P < 0.05, **P < 0.01 by one-way ANOVA with Tukey’s HSD test (B) or Student’s t test (C and D).