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. 2018 Jan 31;115(9):2180–2185. doi: 10.1073/pnas.1718446115

Fig. 2.

Fig. 2.

In vivo induction of MLL-AF4 in iPSC-derived hematopoietic cells enables potent engraftment and multilineage reconstitution. (A) The scheme of transplanting induced iPSC-HSPCs into newborn NSG mice. MA4, MLL-AF4; in vitro diff, in vitro differentiation; BM, bone marrow; PB, peripheral blood. (B) The analysis of human cell chimerism in the bone marrow of recipient mice after 8 wk of transplantation. Transplant groups are as described in A. NT, nontransfection control. (C) Multilineage reconstitution of MLL-AF4 plasmid-treated iPSC-HSPCs in the BM of recipient mice at 8 wk posttransplant. (D) Multilineage reconstitution of human erythroid cells (E; CD235a+ or CD71+), myeloid cells (M; CD33+), B cells (B; CD19+), and T cells (T; CD3+) in the BM, spleen and PB of engrafted mice transplanted with MLL-AF4 plasmid transfected iPSC-HSPCs at 8 wk posttransplant. (E) Total chimerism of human CD45+ cells in the harvested tissues of engrafted mice for each transplant group at 8 wk posttransplant. LV, lentivirus. Data were analyzed for two independent experiments with three mice each time and shown as mean ± SD *P < 0.05.