Fig. 2.

Generation of Liprin-α3–KO mice. (A) CRISPR/Cas9-mediated genome editing in single-cell zygotes. The sgRNA-targeting sequence is shown in bold, the protospacer-adjacent motif (PAM) is red, and the dashed line indicates the deletion. (B) Survival analysis of offspring of heterozygous matings. The dashed line represents a Mendelian distribution (n = 418 animals/54 litters). (C) Liprin-α3 expression in hippocampi analyzed by fluorescent Western blotting (quantification shown in Fig. S3 G and H). The Liprin-α3 antibody 3 cross-reacts with Liprin-α1–α4.