Fig. 2.

Combined cMET blockade and RT shows improved efficacy in schwannoma models. (A) Representative images of immunofluorescent staining of gamma histone H2AX (γH₂AX) in NF2^−/− schwannoma cells treated with control (Ctrl), CRZ (2 μM), RT (8 Gy), or combined CRZ and RT (Combined) for 24 h. (B) Quantification of the number of γH₂AX⁺ foci/nuclei was done using ImageJ software. All assays were performed in triplicate. *P < 0.05; **P < 0.01 (Student t test). (C) Growth curve of Gluc-transduced NF2^−/− tumor in the sciatic nerve model. Growth of nontreated (Ctrl), CRZ (50 mg/kg), 5 Gy irradiated (RT), and Combined tumors was measured by plasma Gluc level tested by a luminometer every 3 d. *P < 0.01. (D) Survival curve of mice bearing NF2^−/− tumors in CPA models (n = 15, n = 14, n = 16, and n = 14 in Ctrl, RT, CRZ, and Combined groups, respectively). Representative of at least three independent experiments, data are presented as mean ± SEM. *P < 0.01; **P < 0.005. (E) Growth of NF2^−/− tumors in mice treated with Ctrl, CRZ, 5 Gy of RT, 10 Gy of RT, or CRZ combined with 5 Gy of RT as measured by Gluc level (n = 10). Representative of at least three independent experiments, all data are presented as mean ± SEM. *P < 0.05.