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. 2018 Feb 13;115(9):E2030–E2039. doi: 10.1073/pnas.1721573115

Fig. 5.

Fig. 5.

Impact of histone chaperone overexpression on silencing. (A) Representative images of wild-type (JRY10790) colonies transformed with the empty-vector control pRS425 or a 2-μm high-copy plasmid containing all three genes of the CAF-1 complex (pCAF-1, alias pJR3418), or ASF1 (pASF1, alias pJR3425), or RTT106 (pRTT106, alias pJR3426). (B) Images of cac1Δ (JRY10803) colonies transformed with the same plasmids as in A. (C) Images of cac2Δ (JRY10815) and cac3Δ (JRY10816) colonies transformed with pRS425 or pCAF-1 (pJR3418). (D) Images of asf1Δ (JRY10804) colonies transformed with the same plasmids as in A. (E) Images of rtt106Δ (JRY10802) colonies transformed with the same plasmids as in A. (F) Plots of the apparent silencing-loss rates of the strains with the indicated mutations transformed with the plasmids described in A and quantified by flow cytometry. See the legend of Fig. 1 for a description of box plots.