FIGURE 4.

Lineage-restricted Csf1r-mApple transgene expression in the lung. (A) Flow cytometric strategy to identify lung leukocytes. (B) Frequency of donor cells within each lung population from tissue protected BM chimeric mice 8 wk after receiving BM from WT (white) or Ccr2^−/− (black) mice. Mean donor chimerism for circulating Ly6C⁺ monocytes is presented as short- or long-dashed lines for recipients of WT or Ccr2^−/− BM, respectively. (C) Expression of Csf1r-mApple in lung leukocytes from a representative WT littermate control (left) and Csf1r-mApple (right) mouse. (D) Frequency of cells expressing Csf1r-mApple and (E) geometric mean fluorescence intensity (GeoMFI) of Csf1r-mApple gated on Csf1r-mApple⁺ cells for different lung leukocytes. Data are from one of two independent experiments. Data presented as mean ± SD of four mice (D and E) or mean ± SEM of five mice (B). The asterisk (*) indicates significant differences using multiple t tests corrected for multiple comparisons using the Holm–Sidak method.