Figure 4.

Identification of extracellular matrix receptor interaction pathway as a possible mechanism of protocadherin‐8 (PCDH8) in gastric cancer. A, Enrichment plot of extracellular matrix receptor interaction pathway regulated by PCDH8 overexpression (P < .01) by gene set enrichment analysis. B, Heat map of genes in extracellular matrix receptor interaction pathway that showed core enrichment with PCDH8 overexpression. C, Relative expression of LAMC2 was significantly upregulated with PCDH8 overexpression in MKN28 and MGC803 cells. D, High expression of LAMC2 was significantly correlated with a shorter disease free survival (left) and tended to correlated with shorter overall survival (right) in the GEPIA cohort. P‐values by log‐rank test are displayed. E, Protein expression of LAMC2 was upregulated when PCDH8 was overexpressed in liver metastatic lesions of nude mice. Upper panel, scale bar, 200 μm; lower panel, scale bar, 50 μm. Values are mean ± SD of 3 replicates. *P < .05; **P < .01; ***P < .001