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. 2018 Jan 27;109(3):699–709. doi: 10.1111/cas.13482

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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HL156A inhibits cell migration and MMP2 and MMP9 expression. A, Wound‐healing assay shows the closure rates of cells treated with HL156A. Images of wound‐closure rates at the indicated time‐points. B, Effect of HL156A on cell migration. Cells were treated with 40 μmol/L HL156A for 22 hours. Migration assays were carried out as described in the Materials and Methods section. Graphs show the quantitative evaluation of the migration rates. The results represent the averages of three independent experiments. C, Expression of MMP2 and MMP9 in HL156A‐treated cells. FaDu and YD‐10B cells were treated with HL156A (40 μmol/L) for the indicated times and subjected to western blotting