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. 2018 Feb 1;109(3):642–655. doi: 10.1111/cas.13478

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Silencing of solute carrier family 35 member F2 (SLC35F2) represses the migration, invasion and colony formation abilities. A, Representative microscopic images of the migration assay in SLC35F2‐KO and control cells. B, Quantification of average migrated cells in control vs SLC35F2 knockout cells. Error bars indicate the mean ± SD based on 3 independent experiments. C, Transwell invasion assays performed using BCPAP or KTC‐1 cells showed decreased invasion capacities by the loss of SLC35F2. D, Numbers of average invaded cells in control vs SLC35F2 knockout cells were shown per test. Representative images (E) and quantification (F) of colonies in colony formation assay. Colonies over 100 cells were scored (**P < .01, ***P < .001)