Figure 2.

Representative cell division associated 1 (CDCA1) peptide‐specific CTL responses. (A) Cultured lymphocytes were subjected to Enzyme‐Linked ImmunoSpot (ELISPOT) assay after depletion of CD4‐positive cells by magnetic beads. (B) TISI cells were incubated with responder cells in the presence of CDCA1 peptide or HIV peptide as an irrelevant control, and the spot counts were quantified. (C) Cultured lymphocytes were analyzed with human leukocyte antigen (HLA)‐A2402/HIV‐dextramer prevaccination (left) or HLA‐A2402/CDCA1‐dextramer (right) combined with anti‐CD8 and ‐CD3 mAbs by flow cytometry. Value of dextramer (+)/CD8(+) cells among CD3(+) cells is shown. R/S, responder/stimulator