Table 3.
ace-1R G119S mutation frequency of An. gambiae (s.l.) from Bouaké and association with phenotypic resistance from carbamate bioassays. Superscript a and b show a significant difference between alive and dead within the same population on a site (P < 0.05). Frequencies were not calculated when the number of individuals tested was low (−)
Phenotype | An. coluzzii | An. gambiae (s.s.) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
n | RR | RS | SS | f (ace1) | n | RR | RS | SS | f (ace-1) | ||
Allokokro | Alive | – | – | – | – | – | 49 | 2 | 44 | 3 | 0.49a |
Dead | – | – | – | – | – | 49 | 0 | 4 | 45 | 0.041b | |
Petessou | Alive | 49 | 0 | 39 | 10 | 0.4a | 1 | – | – | – | – |
Dead | 40 | 0 | 13 | 27 | 0.16b | 3 | – | – | – | – | |
Dar Es Salam | Alive | 9 | 3 | 6 | 0 | 0.67a | 40 | 1 | 36 | 3 | 0.48a |
Dead | 10 | 0 | 2 | 8 | 0.1b | 29 | 0 | 15 | 14 | 0.26b | |
Kennedy | Alive | 2 | – | – | – | – | 23 | 1 | 19 | 3 | 0.46a |
Dead | 4 | – | – | – | – | 21 | 0 | 7 | 14 | 0.17b | |
N’gattakro | Alive | 13 | 0 | 7 | 6 | 0.27 | 12 | 0 | 11 | 1 | 0.46 |
Dead | 4 | 0 | 4 | 0 | 0.5 | 21 | 0 | 9 | 12 | 0.21 |
Abbreviations: f (ace-1), ace-1 G119S mutation frequency within each species, G119S mutated allele from glucine to serine at codon 119 of AChE gene, SS wildtype genotype, RR homozygous mutation, RS heterozygote for the ace-1 gene