Fig. 1.

Pharmacological characterization of SOCE and AA-induced Ca²⁺ entry pathways in BON cells. a Representative traces showing SOCE responses in fura-2 loaded BON cells using standard protocol described in the “Methods” section. Control response is indicated by black trace. The horizontal scale bar indicates 100 s and the vertical scale bar represents a change of 0.05 ratio of fura-2 fluorescence emission (ratio units). Treatment with 30 μM SKF (red trace) or 50 μM 2-APB (blue trace) significantly attenuated this response. b Bar chart showing average CPA-mediated Ca²⁺ release was not altered in response to pharmacological treatments with inhibitors. c Bar chart showing average SOCE in response to treatments described in (a). d Representative traces showing Ca²⁺ entry evoked by application of 6 μM AA in BON cells. Control response is indicated by black trace. Treatment with 30 μM SKF (red trace) significantly inhibited the AA-induced Ca²⁺ entry. In contrast, 50 μM 2-APB (blue trace) enhanced the AA-induced response. The horizontal scale bar indicates 100 s and the vertical scale bar represents a change of 0.5 ratio units. e Bar chart showing averaged amplitudes of AA-induced Ca²⁺ entry in response to indicated treatments. Significance of p < 0.05 and p < 0.01 is indicated by * and **, respectively