Figure 4.

IL-36 stimulates VEGF-A expression and secretion by primary human fibroblasts. (A) Human umbilical vein endothelial cells or foreskin-derived fibroblasts were stimulated with IL-36 (50 ng/ml) for 24 h, before cell lysis. Endothelial cell or fibroblast lysates were processed for detection of VEGF-A protein levels via immunoblot analysis. (B) Quantification of VEGF-A protein levels upon IL-36 stimulation by 2D-densitometry (n = 3). (C) The supernatant from both the stimulated cell types was also tested for VEGF-A protein concentration. Two-way analysis of variance was performed (*p < 0.05).