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Human umbilical vein endothelial cell monolayer was stimulated with or without TNFα 10 ng for 24 h. 1 × 105 monocytes were allowed to adhere to the monolayer for 30 min. Cells were visualised by immunofluorescence microscopy following CD14+ staining (B) and counted (A) (patient monocytes: psoriasis = 8; healthy = 8). Magnification 40×. Unpaired t-test *p < 0.05 and **p < 0.01.
