Figure 6.

(A,B) Supernatants from IL-36γ-stimulated psoriasis or healthy macrophages were used to stimulate human umbilical vein endothelial cell (HUVECs) for 24 h, and ICAM-1 (green) and DAPI (blue) expression as visualised by immunofluorescence microscopy is depicted in panel (A) and intensity of staining measured by fluorescence absorbance summarised in panel [(B); psoriasis n = 8, healthy n = 8]. (C) Healthy and psoriasis monocytes (1 × 10⁵) were allowed to adhere to a HUVEC monolayer for 30 min. The monolayer was stimulated with supernatant derived from IL-36-stimulated/non-stimulated psoriasis or healthy derived macrophage supernatant. Patient monocytes: psoriasis n = 8, healthy n = 8. Unpaired t-test *p < 0.05 and **p < 0.01.