Newly synthesized (D48+ δG9-) perforin traffics from the golgi apparatus to the recycling endosome (RE), a process which is mediated by rab8 and likely VAMP4. The perforin becomes localized with rab11a, rab4, rab37, and vti1b in the RE and is secreted from the RE, which is located in close proximity to the immunological synapse upon stimulation of the CTL[32]. Also shown are the known mechanisms of lytic granule (D48+ δG9+ perforin) trafficking to the immunological synapse, which involves the fusion of a recycling endosome-derived rab11a+ rab27a+ vesicle with rab7+ late endosomes.