Table 2.
Primers in this study
| Primer name | Forward primer sequence (5′–3′) | Rerverse primer sequence (5′–3′) | Purpose | |
|---|---|---|---|---|
| CBF1 | ATGGACGTCGCCGACG | TTAGTCAAACAAATAGCTCCATAGC | Amplification of CBFs | |
| CBF2 | GATGGACGTCGCCGACGC | TTAGTCGAACAAGTAGCTC | ||
| ICE-C | GAAGAA(A/G)GG(T/A/G)ATGCC(T/G)GC(T/C)AAGAA | GG(A/G)CACA(A/G)CTC(T/C)TCCTTGA(C/T)(A/G)CG | Amplification of the conserved sequence of ICEs | |
| ICE-3F | CCAATAGCTTCCTCCCGTCGA | TCGCCTACATCGCGTTCTGGA | 3′ RACE of ICEs | 1st round |
| CTAGACCGCTGGATGGAACCCC | ||||
| ICE-3S | GCCGCATCAAGGAGGAGTTGTG | CTGGAGACCGGCGCAGTGCA | 2nd round | |
| CCTACATCGTGGGATGGAACC | ||||
| ICE-5F | AGGGCGTGATGGAGAACTCGG | TGTGCAGGAGCTCCTTGAGGTA | 5′ RACE of ICEs | 1st round |
| CGATGCTTGATGACGACAGCT | ||||
| ICE-5S | GGGGGCGACTGGGGGTACCT | AAGCCCTGTCCATTTTGCTGATC | 2nd round | |
| CGATGCTTCCCGCTCCTCAACC | ||||
| ICE-IF1 | GCAGAGATAAGTTGCAGTAACTGA | GCGAGCCTCATCCCATCA | Verification of the whole coding sequence of ICEs | 1st round |
| ICE-IS1 | GAACTTGCCAAGAATCCGT | AAAGGAGCGGAACAGCACA | 2nd round | |
| ICE-IF2 | GCTATGCTGTCGCAGTTCAATAG | AAACCATGGGTACTTATTGCAAAT | 1st round | |
| ICE-IS2 | TTGGGTGCAGGAAGGCGA | TTCCTTTGGTATGCTGTCCTCT | 2nd round | |
| COR1 | ATGTCTGGTTGGTTTCAAGAGAAG | TCACCCTCCCATCCCGAG | Amplification of CORs | |
| COR2 | ATGTCGGGTTGGTTCGGGGGTAAC | |||
| 18S | TGAGAAACGGCTACCACATC | TCGGCATCGTTTATGGTTGA | qRT-PCR analysis | |
| CBF4 | AGCCCGAGCAACACTCTTT | GCGCCAAGCTGTCGTAGTA | ||
| CBF9 | GCAGGGTATGCTCGTGTCA | GTCGAACAAGCAGCTCCATA | ||
| ICE41 | AGGGGCAGGCGGTGAACA | GGCGAAGCCATCGAAGCAG | ||
| ICE87 | AAGGCCTTGGTCTTGACGTG | CGCCTTGATTTCCTCAGCCT | ||
| COR1 | TGAGAAAAGCGAGGCTGTCA | TGATCCTTACCGGCTTCCAC | ||
| COR2 | GACCAGACGTTCGGCTTCTT | CGGTCTCCTCGACACACTTT | ||