Figure 1.

Schema for a New Approach to Identify Neoantigen-Specific TCRs

(A) Polyclonal tumor-infiltrating lymphocytes (TILs) were cultured from fresh tumor fragments and screened against tandem minigenes (TMGs) or a peptide library to identify neoantigen-reactive TIL populations. (B) Once a neoantigen-reactive TIL population was identified, this polyclonal TIL population was co-cultured with neoantigen-transfected/pulsed antigen-presenting cells (APCs) for 4 hr, and it was subjected to single-cell RNA-seq sample preparation and sequencing, followed by bioinformatics analysis.