Figure 1.

i.v. Injection of miR-23b Lentivirus Ameliorates Disease Severity

(A) RT-PCR detection of spinal cord miR-23b level in C57BL/6 mice after active immunization. (B) C57BL/6 mice were injected intravenously with 2 × 10⁷ IU/mouse lentivirus 5 days before MOG_35-55 immunization. (C) Cumulative clinical score of each mouse was calculated by summing up the scores of the mouse from day 0 to day 40 p.i. (D and E) Histological examination of inflammatory lesions and demyelination in the lumbar spinal cord of LV-Ctrl- or LV-miR23b-treated mice by (D) H&E staining and (E) Luxol fast blue staining (scale bar, 1 mm) at day 40 p.i. (F and G) Statistical analysis of (D) is in (F) and (E) is in (G). Data represent three independent experiments. Data are mean ± SD (n = 10 each group). **p < 0.01 and ***p < 0.001, determined by one-way ANOVA (A), unpaired Student’s t test (B, C, and G), or nonparametric test (F).