Figure 3.

LV-miR23b Limits CNS-Directed T Lymphocyte Extravasation

(A) Immunohistochemistry staining of CD45⁺ cells in spinal cord of LV-Ctrl- and LV-miR23b-treated mice (day 17 p.i.). (B) Statistical analysis of (A). (C) Spinal cords and brains were harvested and MNCs isolated (n = 5 each group). Total MNC numbers in the CNS were counted under light microscopy. (D–H) Percentages of (D) CD4⁺ and (E) CD8⁺ T cells among MNCs and (F) IFN-γ⁺, (G) IL-17⁺, and (H) GM-CSF⁺ cells among CD4⁺ T cells were determined by flow cytometry. (I) Absolute numbers of different subtypes of CNS-infiltrating cells were calculated by multiplying the percentages of these cells by total numbers of MNCs obtained from each spinal cord of mice. Data are mean ± SD (n = 5 each group). **p < 0.01 and ***p < 0.001, determined by one-way ANOVA (A) or unpaired Student’s t test.