FIGURE 1.

Time course of plasma cocaine hydrolysis activities in mice receiving viral gene transfer of mouse or human BChE mutated for enhanced cocaine hydrolysis (CocH by i.v. injection of standard AAV). Data are from repeated samples of mouse plasma across time (weeks to months as noted). CocH activity was measured by enzymatic release of tritium-label isolated by solvent separation (Brimijoin et al., 2002). (A) Mice given AAV gene transfer of mouse CocH. Levels of expression were modest but were maintained for more than 1 year. (B) Mice given gene transfer of human CocH. Activities were higher, but much shorter lived (Geng et al., 2013, Figure 2).