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. 2018 Feb 27;9:377. doi: 10.3389/fimmu.2018.00377

Figure 2.

Figure 2

Valproic acid (VPA) and zoledronate (ZOL) upregulated the level of γδ T cells cytotoxicity-related indicators against osteosarcoma cells. HOS and U2OS cells were treated for 24 h with control, 1 mM VPA, 2 µM ZOL, and VPA + ZOL, respectively, before 2 h co-cultured with γδ T cells at an E:T ratio of 5:1. (A,B) CD107a expression levels on γδ T cells were measured by flow cytometry. MFI of CD107a+ γδ T cells was presented in histograms. (C,D) Perforin expression levels of γδ T cells were measured by flow cytometry. MFI of perforin+ γδ T cells was presented in histograms. (E,F) IFN-γ expression levels of γδ T cells were measured by flow cytometry. MFI of IFN-γ+ γδ T cells was presented in histograms. Red histogram line was isotype control and panels were overlapped. All the values were shown as mean ± SD from three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001 versus control, #p < 0.05.