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. 2018 Feb 27;9:45. doi: 10.3389/fgene.2018.00045

Table 1.

Firefly luciferase reporters used in HeLa and NIH 3T3 cells.

Cells Reporter name Promoter miRNA sites 3′ UTR Type
HeLa let-7_3xP PGK 1x let-7B, 2xlet-7P BGH_polyA Stable clone
let-7_4xB PGK let-7_4xB BGH_polyA Stable pool
miR-30_1xP SV40 miR-30_1xP SV40_polyA Transient transfection
miR-30_4xB PGK miR-30_7xB BGH_polyA Stable pool
miR-30_4xM PGK miR-30_4xM BGH_polyA Transient transfection
NIH 3T3 let-7_3xP PGK 1x let-7B, 2xlet-7P BGH_polyA Stable clone
let-7_4xB PGK let-7_4xB BGH_polyA Transient transfection
let-7_4xM PGK let-7_4xM BGH_polyA Transient transfection
miR-30_4xB PGK miR-30_7xB BGH_polyA Transient transfection
miR-30_1xP SV40 miR-30_1xP SV40_polyA Transient transfection
Luciferase SV40 No SV40_polyA Transient transfection