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Resveratrol attenuates TM-induced ER stress in HT22 cells. HT22 cells were treated with 100 ng/ml TM with or without 50 μM resveratrol for 24 h. The expression of GRP78 protein was detected by immunofluorescence staining (A) and calculated (B). The mRNA levels of XBP1S was examined by RT-PCR (C). The expression of CHOP, p-JNK and cleaved-caspase-12 was detected by western blot (D–G). Data are shown as mean ± SEM. #p < 0.05 vs. Control. *p < 0.05 vs. TM. Scale bar = 20 μm.
