Figure 6.

Sirt3 activation contributes to resveratrol-induced autophagy regulation. HT22 cells were transfected with Si-control or Si-Sirt3 for 48 h, and the expression of Sirt3 protein was detected by immunofluorescence staining (A) and calculated (B). After transfection, HT22 cells were treated with resveratrol for another 24 h, and the expression of Beclin1 and LC3 was detected by western blot (C–E). Immunofluorescence staining of LC3II was performed to assess autophagy in HT22 cells (F), and the number of LC3-positve puncta was counted (G). Data are shown as mean ± SEM. ^#p < 0.05 vs. Control. ^*p < 0.05 vs. Si-control. Scale bar = 20 μm.