Figure 7.

Knockdown of Sirt3 partially prevents resveratrol-induced protection. HT22 cells were transfected with Si-control or Si-Sirt3 for 48 h, and treated with TM in the presence or absence of resveratrol for another 24 h. Cell viability was measured by MTT assay (A), and cytotoxicity was determined by LDH assay (B). The expression of cleaved-caspase-12 was detected by western blot (C). The mRNA levels of XBP1S was examined by RT-PCR (D). The expression of CHOP, p-JNK, and cleaved-caspase-12 was detected by western blot (E–G). Data are shown as mean ± SEM. ^#p < 0.05 vs. Control. ^*p < 0.05.