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. 2017 Dec 5;26(2):524–541. doi: 10.1016/j.ymthe.2017.11.019

Figure 2.

Figure 2

Triple ED-AAV Vectors Efficiently Transduce ED In Vitro

(A and B) Western blot (WB) analysis of lysates from HEK293 cells either transfected with pED or infected with triple AAV2/2 vectors encoding for ED and stained with α-3xflag (A) and α-EGFP (B) antibodies. The arrows on the right indicate the following protein products: #A, ED full-length protein; #B, from AAV 2 + 3; #C, from AAV 1 + 3; #F, from AAV 3; #D and #E, aggregates of AAV 3. α-3xflag, WB with anti-3xflag antibodies; α-EGFP, WB with anti-EGFP antibodies; α-β-Tubulin, WB with anti-β-Tubulin antibodies, used as loading control. Neg, not infected cells. The molecular weight ladder is depicted on the left; 20 and 200 μg of proteins for transfected and infected samples, respectively, were loaded. The WB images are representative of n = 4 independent experiments. (C) Fluorescent analysis of HEK293 cells infected with triple AAV2/2 vectors encoding for ED. The scale bar (200 μm) is depicted in the figure. DsRed, Discosoma red fluorescent protein; Merge: overlay of EGFP and DsRed images.