Figure 3.

Transcriptomic Analysis following Transduction with Triple AAV Vectors

(A) Schematic representation of triple CDH and ALMS-AAV vectors with primers used for real-time qPCR (A→F). Prom, promoter; CDS, coding sequence; polyA, polyadenylation signal; 3xflag, triple flag tag; SD, splicing donor signal; SA, splicing acceptor signal; rec, recombinogenic region. (B) Results of the real-time qPCR analysis performed on cDNAs from HEK293 cells or mouse eyecups treated with either CDH- or ALMS-AAV 1 + 2 + 3 vectors. The left column shows the vector assembly predicted on the basis of the set of primers used. The two columns on the right show the abundance of the PCR products relative to full-length detected by the D + E primers. Only products with a relative abundance >0.1 are shown. Highlighted in italic is the relative abundance of PCR products resulting from the desired AAV directional concatemerization. Highlighted in bold are the PCR products with a relative abundance >1.