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. 2017 Dec 5;26(2):524–541. doi: 10.1016/j.ymthe.2017.11.019

Figure 4.

Figure 4

Triple CDH- and ALMS-AAV Vectors Efficiently Transduce CDH23 and ALMS1 In Vitro

(A and B) WB analysis of lysates from HEK293 cells infected with triple AAV2/2 vectors encoding for CDH23 and incubated with either anti-3xflag (A) or anti-CDH23 (B) antibodies. The arrows on the right indicate the following protein products: #A, full-length CDH23 protein; #B, from AAV 3. (C and D) WB analysis of lysates from HEK293 cells infected with triple AAV2/2 vectors encoding for ALMS1 and incubated with either anti-3xflag (C) or anti-ALMS1 (D) antibodies. The arrows on the right indicate the following protein products: #A, full-length ALMS1 protein; #B, from AAV 1 + 3. α-3xflag, WB with anti-3xflag antibodies; α-CDH23, WB with anti-CDH23 antibodies; α-ALMS1, WB with anti-ALMS1 antibodies; α-Filamin A, WB with anti-Filamin A antibodies, used as loading control. Neg, cells infected with control AAV2/2-CMV-EGFP vectors. The molecular weight ladder is depicted on the left, 100 (A), 250 (B), or 200 μg (C and D) of proteins were loaded.