Skip to main content
. 2017 Dec 5;26(2):524–541. doi: 10.1016/j.ymthe.2017.11.019

Figure 6.

Figure 6

Triple AAV Vectors Drive Full-Length Protein Expression in the Pig Retina

(A) Western blot (WB) analysis of lysates from Large White pig retina 2 months following subretinal injection of either single or triple AAV2/8 vectors encoding for ED under the control of the PR-specific promoter IRBP. The arrow indicates the full-length protein. (B) WB analysis of truncated products in lysates from Large White pig retina 2 months following subretinal injection of the combinations of triple ED-AAVs. Lysates of HEK293 cells infected with an ED-AAV 1 + 2 + 3 are shown on the left as positive control. The arrows indicate the following products: #A, ED full-length protein; #E, probably from AAV 3. α-3xflag, WB with anti-3xflag antibodies; α-β-Actin, WB with anti-β-Actin antibodies, used as loading control. Neg, lysates of retinas following injection with PBS. The molecular weight ladder is depicted on the left; 200 μg of proteins were loaded.