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. 2017 Dec 5;26(2):524–541. doi: 10.1016/j.ymthe.2017.11.019

Figure 7.

Figure 7

Subretinal Administration of Triple AAV Vectors Results in Full-Length CDH23 and ALMS1 Protein Expression in the Mouse Retina

(A) Western blot (WB) analysis of eyecup lysates from C57BL/6J mice 3 months following subretinal injection of triple AAV2/8 encoding for CDH23 under the control of the ubiquitous CMV promoter. The arrows on the right indicate the following products: #A, full-length proteins; #C, probably from AAV 1 + 3. (B) WB analysis of eyecup lysates from C57BL/6J mice 3 months following subretinal injection of triple AAV2/8 encoding for ALMS1 under the control of the ubiquitous short CMV promoter. The arrow on the right indicates full-length ALMS1. α-3xflag, WB with anti-3xflag antibodies; α-Dysferlin, WB with anti-Dysferlin antibodies, used as loading control. Neg, lysates of eyecups following injection with PBS. The molecular weight ladder is depicted on the left; 200 μg of proteins (A) or the whole-eyecup lysates (B) were loaded.