Figure 8.

Alms1^−/− Retinal Structure and Function following Subretinal Delivery of Triple AAV Vectors

(A) Immunohistochemical (IHC) analysis with anti-3xflag antibodies of retinal cryosections from C57BL/6J mice 2 months following subretinal injections of either triple AAV2/8 encoding for ALMS1 under the control of the PR-specific GRK1 promoter (top panels) or PBS (bottom panels). The pictures are representative of n = 4 injected eyes. The scale bar (20 μm) is depicted in the figure. Merge, overlay of DAPI and ALMS1. ONL, outer nuclear layer; OS, outer segment. (B) Spectral domain optical coherence tomograms (SD-OCT) analysis of Alms1^−/− mice injected subretinally with either triple GRK1-ALMS-AAV vectors (white bars) in one eye or PBS in the contralateral eye (black bars). Results are reported as means ± SE. (C and D) Electroretinographic analysis of a-wave (C) and b-wave (D) light responses of Alms1^−/− mice injected subretinally with either triple GRK1-ALMS-AAV vectors (white bars) or PBS in the contralateral eye (black bars). Results are reported as means ± SE. Light intensity of 20 cd s/m² (a-wave); background white light of 50 cd s/m² and light intensity of 20 cd s/m² (b-wave).