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. 2018 Feb 27;11:59. doi: 10.3389/fnmol.2018.00059

Figure 2.

Figure 2

Genistein induces proline-rich membrane anchor (PRiMA)-linked acetylcholinesterase (AChE) in cultured PC12 cells. (A) Cultured PC12 cells were treated with genistein for 48 h, and NGF (20 ng/ml) served as a positive control. Cell lysates were collected for Ellman assay (left panel) and sucrose density gradient analysis (right panel). (B) Protein lysates of the cultures as in (A) were performed Western blot analysis (left panel). The expression level of proteins was calculated. Quantification plot was shown in histograms (right panel). (C) Total RNAs was extracted from cultures to perform quantitative PCR. Values were normalized with 18S rRNA. (D) Cultured PC12 cells were transiently transfected with two promoter constructs, pAChE-Luc and pPRiMA-Luc for 24 h. Cell lysates were collected for luciferase assay. Values are expressed as the fold, or percentage, of increase to basal reading, and are in means ± SEM, n = 5, each with triplicate samples. *p < 0.05; **p < 0.01; ***p < 0.001.