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. 2018 Feb 27;11:59. doi: 10.3389/fnmol.2018.00059

Figure 6.

Figure 6

E2 induces the transcription of AChE. (A) Cultured PC12 cells were transiently transfected with pAChE-Luc for 24 h and pre-treated with or without G15 (10 μM). Then, treated with E2 for 48 h. Cell lysates were collected for luciferase assay (left panel). Total RNAs was extracted from cultures to determine the mRNA levels of AChE and PRiMA (right panel). Values were normalized with 18S rRNA. (B) Cultured PC12 cells were transiently transfected with pCRE-Luc or pERE-Luc for 24 h and pre-treated with or without G15 (10 μM). Then, E2 was treated for 2 days. Cell lysates were collected for luciferase assay. (C) Cultured PC12 cells were transiently transfected with pCRE-Luc for 24 h and pre-treated with or without G15 (10 μM). Then, treated with G1 for 48 h. Cell lysates were collected for luciferase assay. Data are expressed as the percentage of increase where control value is set as 0. mean ± SEM, n = 5, each with triplicate samples. *p < 0.05; **p < 0.01; ***p < 0.001.