Figure 2.

Key steps of the pancreas digestion protocol. (A) Injection of cold Col-EGTA buffer in the common bile duct (black arrows). (B) Pancreas (dotted lines) blows and becomes white. (C) Comparison between injected and no-injected pancreas. (D) Pancreas takes a floppy texture. (E) Pancreas is cut into small pieces. (F) Pancreas is partially digested with Col-Ca²⁺ buffer and small pieces are still present. (G) First pellet of dissociated cells that must be kept on ice. (H) Col-Ca²⁺ buffer is setback on remaining undigested pancreas. (I) Digestion solution takes a milky appearance. (J) Final pellet of cell that must be kept on ice. I, Intestine; L, Liver; P, Pancreas; S, Spleen.