Figure 4.

Immune Profiles of Effector and Suppressive T Lymphocytes among the TILs of Index and Distant Tumors from Mice Treated with Various Protocols of Local Irradiation with or without the CTGF/E7 DNA Vaccine

(A) Representative images showing H&E staining of infiltrated leukocytes in the index tumors of different experimental groups (n = 5 per group). Arrows indicate infiltrated leukocytes. (B) Representative images showing immunofluorescence staining of infiltrated CD8⁺ cells in the index tumors of different experimental groups (n = 5 per group). Blue, DAPI staining; red, CD8. (C–E) Bar figures indicating the percentages of CD8⁺ cytotoxic T lymphocytes among the TILs of the index tumors (C), the percentages of CD8⁺ cytotoxic T lymphocytes among the TILs of the distant tumors (D), and the percentages of Tregs among the TILs of the index tumors (E) from various experimental groups (n = 5 per group). Data are shown as mean ± SD. (F) Bar figures indicating the ratio of cytotoxic T cells to Tregs within the index tumors from various groups (n = 5 per group). Data are shown as mean ± SD.