Skip to main content
. 2017 Nov 21;26(2):404–419. doi: 10.1016/j.ymthe.2017.11.011

Figure 6.

Figure 6

Irradiation Promotes DC Maturation and Enhances Antigen-Specific T Cell Immunity through Apoptosis-Related Molecules

(A) Bar figures indicating the percentages of apoptotic TC-1 tumor cells after treatment with 3, 6, or 12 Gy of radiation or without irradiation at the indicated intervals (0, 24, and 48 hr) (n = 3 per group). Data are shown as mean ± SD. (B) Bar figures indicating the percentages of HMGB-1 (B1), CRT (B2), annexin A1 (B3), and HSP70 (B4)-expressing TC-1 tumor cells with or without irradiation treatment (3, 6, or 12 Gy) at the indicated intervals (0, 24, and 48 hr) (n = 3 per group). Data are shown as mean ± SD. (C) Bar figures indicating the percentages of mature DCs after pulsing with irradiated TC-1 tumor cells (n = 3 per group). Data are shown as mean ± SD. (D) Representative figures showing immunofluorescence of the TC-1-LG tumor cells co-cultured with naive T cells or with E7-specific cytotoxic T cells pulsed with BMM-derived DCs that were cultured first with irradiated TC-1 tumor cells (3, 6, or 12 Gy) (n = 3 per group). Detection was performed using an IVIS Imaging system. (E) Bar figures indicating the luminescence of TC-1-LG tumor cells in various groups (n = 3 per group). Data are shown as mean ± SD.