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. Author manuscript; available in PMC: 2018 Mar 4.
Published in final edited form as: Dev Biol. 2017 Mar 18;430(2):385–396. doi: 10.1016/j.ydbio.2017.03.010

Fig. 4.

Fig. 4

EVL Ca2+ signaling pattern in ventralized and dorsalized embryos. (A-B) Automated quantification of EVL Ca2+ transient frequency in ichabod/β-catenin2 ventralized embryos between 2.5 hpf and 6.5 hpf. Error bars represent S.E.M.; N=6 embryos. (C) Automated quantification of EVL Ca2+ transient frequency in β-catenin1-injected dorsalized embryos. Induced dorsal organizer is oriented to the right. Error bars represent S.E.M. *, P≤0.05. **, P≤0.01***, P≤0.001. ****, P≤0.0001; N=6 embryos. (D) Comparison of Ca2+ transient number in individual quadrants among WT, ventralized, and dorsalized embryos at 3.5–4 hpf. Error bars represent standard deviation. ns, not significant. *, P≤0.05. ****, P≤0.0001; (E) Representative 30-min time-lapse overlay of GCaMP6s signals in WT, ventralized, and dorsalized embryos from 2.5 hpf to 6.5 hpf.